Nonprotein sulfhydryl compounds in canine gastric mucosa: effects of PGE2 and ethanol

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Abstract
By use of an in vivo canine chambered stomach preparation in which the gastric mucosa was partitioned into 2 equal halves, the effect of topical 16,16-dimethyl [prostaglandin] PGE2 (DMPGE2) (1 .mu.g/ml of perfusate) and 8% and 40% ethanol on tissue levels of nonprotein sulfhydryl compounds were assessed. Both DMPGE2 and 8% ethanol significantly increased (P < 0.005) mucosal levels of nonprotein sulfhydryls when compared with corresponding mucosa bathed with saline alone. Mucosa bathed with 40% ethanol showed significantly decreased levels. If mucosa was bathed with DMPGE2 or 8% ethanol prior to exposing the stomach to 40% ethanol, this depletion in sulfhydryl compounds was not observed. Since exogenously administered PG and mild irritants (such as low-dose alcohol) can prevent gastric mucosal damage by necrotizing agents (such as high-dose alcohol), nonprotein sulfhydryls may play a role in mediating mucosal protection.