A Novel Pyrrolidinyl PNA Showing High Sequence Specificity and Preferential Binding to DNA over RNA
- 18 July 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 124 (32) , 9326-9327
- https://doi.org/10.1021/ja012543u
Abstract
A novel conformationally constrained pyrrolidinyl peptide nucleic acid (PNA) carrying an d-aminopyrrolidine carboxylic acid (d-Apc) spacer was synthesized, and its interactions with complementary oligo- and polynucleotides were studied by UV and CD spectroscopy. The decathymine PNA formed very stable PNA−DNA complexes with poly(dA) and (dA)10 by a sequence-specific A-T pairing. The interaction with poly(rA) gave the corresponding PNA−RNA complex with much lower stability.Keywords
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