DNA electrotransfer: its principles and an updated review of its therapeutic applications
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- 29 September 2004
- journal article
- review article
- Published by Springer Nature in Gene Therapy
- Vol. 11 (S1) , S33-S42
- https://doi.org/10.1038/sj.gt.3302367
Abstract
The use of electric pulses to transfect all types of cells is well known and regularly used in vitro for bacteria and eukaryotic cells transformation. Electric pulses can also be delivered in vivo either transcutaneously or with electrodes in direct contact with the tissues. After injection of naked DNA in a tissue, appropriate local electric pulses can result in a very high expression of the transferred genes. This manuscript describes the evolution in the concepts and the various optimization steps that have led to the use of combinations of pulses that fit with the known roles of the electric pulses in DNA electrotransfer, namely cell electropermeabilization and DNA electrophoresis. A summary of the main applications published until now is also reported, restricted to the in vivo preclinical trials using therapeutic genes.Keywords
This publication has 139 references indexed in Scilit:
- High-level expression of interleukin-4 following electroporation-mediated gene transfer accelerates Type 1 diabetes in NOD miceJournal of Autoimmunity, 2003
- Regression of tumors by IFN-α electroporation gene therapy and analysis of the responsible genes by cDNA arrayGene Therapy, 2002
- Transfer of bcl-xs plasmid is effective in preventing and inhibiting rat hepatocellular carcinoma induced by N-nitrosomorpholineGene Therapy, 2001
- Gene Therapy for the Treatment of Hemophilia B Using PINC-Formulated Plasmid Delivered to Muscle with ElectroporationMolecular Therapy, 2001
- Long-term production of erythropoietin after electroporation-mediated transfer of plasmid DNA into the muscles of normal and uremic ratsGene Therapy, 2001
- Determination of Optimal Parameters for in Vivo Gene Transfer by Electroporation, Using a Rapid in Vivo Test for Cell PermeabilizationBiochemical and Biophysical Research Communications, 1999
- In vivo gene electroinjection and expression in rat liverFEBS Letters, 1996
- Electrochemotherapy of spontaneous mammary tumours in miceEuropean Journal of Cancer and Clinical Oncology, 1991
- Electrochemotherapy potentiation of antitumour effect of bleomycin by local electric pulsesEuropean Journal of Cancer and Clinical Oncology, 1991
- Electric field mediated transformation: Isolation and characterization of a TK+ subcloneBiochemical and Biophysical Research Communications, 1985