HLA‐DQB1 allele typing by a new PCR‐RFLP method: Correlation with a PCR‐SSO method
- 1 September 1992
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 40 (3) , 116-123
- https://doi.org/10.1111/j.1399-0039.1992.tb02102.x
Abstract
We have developed a new PCR‐RFLP method for HLA‐DQB1 typing. This method was easy to follow, requiring only one DQB1 generic amplification and 5 endonucleases to assign 14 out of 15 HLA‐DQB1 alleles. In addition, we determined that by using one generic amplification and two enzymes (Sau96 I and Hae III) it was possible to type the generic specificities: DQw2, DQw4, DQw5, DQw6, and DQw7, DQw8‐9, providing a practical alternative for serological HLA‐DQ generic typing. We also performed a side‐by‐side correlation with a PCR‐SSO typing method and found an almost 100% concordance between the methods. The limitations of these methods were: 1) the PCR‐RFLP method did not allow the differentiation between the HLA‐DQB1 *0602 and *0603 alleles; 2) the PCR‐SSO method gave crosshybridization signals in the detection of *0302 or *0303 alleles. Our results suggested that both methods, PCR‐RFLP and PCR‐SSO, are useful alternatives for HLA‐DQB1 typing.Keywords
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