Fluoride is a strong and specific inhibitor of (asymmetrical) Ap4A hydrolases
- 26 March 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 262 (2) , 205-208
- https://doi.org/10.1016/0014-5793(90)80190-t
Abstract
Fluoride acts as a noncompetitive, strong inhibitor of (asymmetrical) Ap4A hydrolases (EC 3.6.1.17). The K i values estimated for the enzymes isolated from seeds of some higher plants (yellow lupin, sunflower and marrow) are in the range of 2–3 μM and I 50 for the hydrolase from a mammalian tissue (beef liver) is 20 μM. The anion, up to 25 mM, does not affect the following other enzymes which are able to degrade the bis(5'-nucleosidyl)-oligophosphates: Escherichia coli (symmetrical) Ap4A hydrolase (EC 3.6.1.41), yeast Ap4A phosphorylase (EC 2.7.7.53), yellow lupin Ap3A hydrolase (EC 3.6.1.29) and phosphodiesterase (EC 3.1.4.1). None of halogenic anions but fluoride affects the activity of (asymmetrical) Ap4A hydrolases. Usefulness of the fluoride effect for the in vivo studies on the Ap4A metabolism is shortly discussed.Keywords
This publication has 31 references indexed in Scilit:
- Synthesis of diadenosine 5',5'''-P1,P4-tetraphosphate (AppppA) from adenosine 5'-phosphosulfate and adenosine 5'-triphosphate catalyzed by yeast AppppA phosphorylaseBiochemistry, 1988
- Yeast diadenosine 5',5'''-P1,P4-tetraphosphate .alpha.,.beta.-phosphorylase behaves as a dinucleoside tetraphosphate synthetaseBiochemistry, 1987
- Catabolism of Ap3A and Ap4A in human plasma. Purification and characterization of a glycoprotein complex with 5'-nucleotide phosphodiesterase activityEuropean Journal of Biochemistry, 1985
- Nucleotide pyrophosphatase from potato tubersEuropean Journal of Biochemistry, 1984
- Dinucleosidetetraphosphatase inhibition by Zn(II)Biochemical and Biophysical Research Communications, 1983
- Isolation and characterization of diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase from Physarum polycephalumBiochemistry, 1982
- Dinucleosidetriphosphatase from Rat Liver. Purification and PropertiesEuropean Journal of Biochemistry, 1977
- Diguanosinetetraphosphatase from Rat Liver: Activity on Diadenosine Tetraphosphate and Inhibition by Adenosine TetraphosphateEuropean Journal of Biochemistry, 1975
- Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reactionBiochemical Pharmacology, 1973
- Enzymatic synthesis of diadenosine tetraphosphate and diadenosine triphosphate with a purified lysyl-sRNA synthetaseBiochemical and Biophysical Research Communications, 1966