Synthesis of Anisolylated Aspartyl and Glutamyl Tripeptides

Abstract
A process has been developed for transforming the β-carboxyl of aspartate and the γ-carboxyl of glutamate into anisolyl ketones. These ketones are occasional byproducts in peptide synthesis, resulting from deprotection or resin-removal processes in the presence of anisole as a scavenger. The ketone amino acids have been incorporated in a tripeptide by coupling with CBMIT. During peptide bond formation the keto group of the glutamyl residue required protection, which was provided as the ethylene dithioketal.

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