Genomic Structure of the Human Proliferating Cell Nucleolar Protein P120

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Abstract
A gene for human proliferating cell nucleolar protein p120 has been isolated from a human genomic library using p120 cDNA as a probe. The gene spanned 12 kilobase pairs and was composed o f 15 exons and 14 introns. Unusual splice junction sequences, (AT) and (AC), instead of (GT) and (AG), respectively, were located at the splice sites for intron F. The 5′ flanking region was analyzed for putative cis-acting factor binding sites. The region contained a TATA-like sequence and the CCAAT box. CAT assays indicated that the region −2532/+102 was necessary for transcription. Two specific areas at −1444/−1224 and −537/−278 were shown to be important for transcription. Two “GC" boxes and two “GC"-rich sequences were observed. Other sites upstream o f these sites were analyzed for homology to other gene control regions.