Separation of Molecular Species of Glucosylceramide by High Performance Liquid Chromatography of Their Benzoyl Derivatives
- 1 November 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 80 (5) , 1181-1183
- https://doi.org/10.1093/oxfordjournals.jbchem.a131375
Abstract
The method of separation of glucosylceramide by HPLC was reported. Glucosylceramide was perbenzoylated and separated on a packed μBondapack C18 column, using methanol as eluting solvent. The pattern obtained by HPLC closely resembled that obtained by GLC of the TMS-glucosylceramide, and reflected the molecular species of fatty acid components. This method is reproducible, and sensitive as GLC. This method also can be used for analysis of higher glycolipids.This publication has 2 references indexed in Scilit:
- Quantitative analysis of brain galactosylceramides by high performance liquid chromatography of their perbenzoyl derivativesJournal of Lipid Research, 1976
- The separation of the C12-C18 fatty acids by reversed-phase partition chromatographyBiochemical Journal, 1950