20α‐Hydroxysteroid dehydrogenase expression in hemopoietic cell cultures and its relationship to interleukin 3

Publisher Website
Google Scholar
Abstract
Expression of 20α‐hydroxysteroid dehydrogenase (20α‐SDH) has been investigated in long‐term bone marrow cultures derived from normal and nude mice in the presence of horse serum and hydrocortisone. 20α ‐SDH expression rises markedly in the adherent cells derived from normal bone marrow. Early (1‐5 days) lipid profiles are similar to those seen in T cells, but at later times (6‐14 days) stromal‐type lipid patterns dominate. Similar culture of fetal liver and nu/nu bone marrow cells shows rises in 20α ‐SDH expression, in situations where T cells are notably absent. The expression of 20α‐SDH is not associated with detectable endogenous production of “interleukin 3” activity, and although addition of “IL 3” to bone marrow cultures increases the levels of 20α ‐SDH, this is associated with granulopoiesis rather than lymphoid development.