Über die Flügelpigmente der Schmetterlinge. XIII. Nachweis und Bestimmung von Leukopterin

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Abstract
Leucopterin is an oxidation product of xanthopterin. To study the significance of leucopterin in animal N metabolism, two 1-g. samples (A and B) of excreta from clothes moth caterpil-lars are extracted with ether and methanol, heated with 40 ml. N NaOH and centrifugalized. To the supernatant soln. A is added 0.4 g. leucopterin and both supernatant solns. are treated with dilute HC1. After standing several hrs., the mixtures are filtered, the residues dissolved in 20 ml. N NaOH, heated with 1 g. animal charcoal and filtered. Leucopterin has a blue fluorescence in alkaline soln., which is measured in the Zeiss-Pulfrich photometer, and the amt. of leucopterin present then detd. by a standard reference curve. The excreta of the clothes moth cate-pillar contain 0.01% leucopterin. One part of leucopterin is soluble in 10[degree] parts of water at 20[degree]. No leucopterin is found in human urine, snake excreta or guano.

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