Isolation of maxicircle component of kinetoplast DNA from hemoflagellate protozoa.
- 1 April 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (4) , 1585-1588
- https://doi.org/10.1073/pnas.76.4.1585
Abstract
Maxicircle DNA has been isolated from the kinetoplast DNA of Leishmania tarentolae culture forms by bouyant separation in CsCl in the presence of the A + T-binding dye Hoechst 33258, after liberation from the kinetoplast DNA network by cleavage with the single-hit restriction endonuclease EcoRI. The purified linearized maxicircle DNA has a density in CsCl of 1.681 g/cm3 (79% A + T) and a molecular weight of approximately 18-20 x 10(6). The maxicircle molecule exhibited intramolecular base composition heterogeneity ranging from 85% A + T to 74% A + T.Keywords
This publication has 28 references indexed in Scilit:
- Isolation and characterization of kinetoplast DNA and RNA of Phytomonas davidiPlasmid, 1978
- Isolation of Inc P-2 plasmid DNA from Pseudomonas aeruginosaPlasmid, 1978
- Maxi-circles in the kinetoplast DNA of Trypanosoma megaExperimental Cell Research, 1977
- Comparison of various ultraviolet sources for fluorescent detection of ethidium bromide-DNA complexes in polyacrylamide gelsAnalytical Biochemistry, 1977
- Characterization of kinetoplast DNA networks from the insect trypanosome Crithidia luciliaeBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977
- Heterogeneity of the kinetoplast DNA molecules of Trypanosoma cruziBiochemistry, 1977
- A simplified method for preparation of mouse satellite DNAAnalytical Biochemistry, 1977
- Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.Proceedings of the National Academy of Sciences, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Nucleotide sequence of bacteriophage φX174 DNANature, 1977