Ornithine Decarboxylase and Trypanothione Reductase Genes in Leishmania braziliensis guyanensis
- 1 September 1989
- journal article
- research article
- Published by Wiley in The Journal of Protozoology
- Vol. 36 (5) , 498-501
- https://doi.org/10.1111/j.1550-7408.1989.tb01085.x
Abstract
Ornithine decarboxylase and trypanothione reductase are the key enzymes in polyamine and trypanothione metabolism in kinetoplastids. Using a heterologous Trypanosoma brucei brucei probe for ornithine decarboxylase and a mixed synthetic probe of 29 oligonucleotides for trypanothione reductase, we have detected the putative genes for these enzymes by Southern blot hybridization using genomic DNA of Leishmania braziliensis guyanensis MHOM/SR/80/CUMC 1. The trypanothione reductase probe was constructed both from the conserved condon usage of the redox active site for other flavin oxidoreductases over a wide evolutionary scale, and the preferred codon usage for other genes in species of Leishmania.Keywords
This publication has 18 references indexed in Scilit:
- Beta tubulin gene of the parasitic protozoan Leishmania mexicanaMolecular and Biochemical Parasitology, 1988
- Trypanothione reductase of Trypanosoma congolense: gene isolation, primary sequence determination, and comparison to glutathione reductaseBiochemistry, 1988
- Biochemical changes associated with α-difluoromethylornithine uptake and resistance in Trypanosoma bruceiMolecular and Biochemical Parasitology, 1987
- Complete Amino Acid Sequence of Human Ornithine Decarboxylase Deduced from Complementary DNADNA, 1987
- In vivo effects of difluoromethylornithine on trypanothione and polyamine levels in bloodstream forms of Trypanosoma bruceiMolecular and Biochemical Parasitology, 1987
- Effects of DL‐Alpha‐Difluoromethylornithine on Leishmania donovani Promastigotes1The Journal of Protozoology, 1986
- Purification and characterization of trypanothione reductase from Crithidia fasciculata, a new member of the family of disulfide-containing flavoprotein reductasesBiochemistry, 1986
- Karyotype Analysis of Leishmania Species and Its Use in Classification and Clinical DiagnosisScience, 1986
- Glutathione reductase from Escherichia coli: cloning and sequence analysis of the gene and relationship to other flavoprotein disulfide oxidoreductasesBiochemistry, 1986
- Deoxyribonucleic acid sequence of a gene from the Pseudomonas transposon TN501 encoding mercuric reductaseBiochemistry, 1983