Synthesis of human β‐endorphinyl‐thiolglycine and its use for the preparation of affinity columns of β‐endorphin
- 1 November 1982
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 20 (5) , 429-433
- https://doi.org/10.1111/j.1399-3011.1982.tb03063.x
Abstract
The peptide human .beta.-endorphinyl-thiolglycine (I) was synthesized by the solid-phase method. The citraconyl derivative of peptide I was coupled to aminohexyl-Sepharose by reaction with silver nitrate/N-hydroxysuccinimide in water. The citraconyl groups were removed in aqueous acetic acid and the resulting resin was used in affinity chromatograpy for the purification of antisera to .beta.-lipotropin and .beta.-endorphin.Keywords
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