DIFFERENTIAL ENDOTOXIN SENSITIVITY OF LYMPHOCYTES AND MACROPHAGES FROM MICE WITH AN X-LINKED DEFECT IN B-CELL MATURATION

  • 1 January 1978
    • journal article
    • research article
    • Vol. 121  (2) , 685-690
Abstract
The in vitro sensitivity of B [bone marrow derived] lymphocytes and macrophages derived from (CBA/N .times. DBA/2N) F1 male mice, which carry an X-linked recessive gene that produces defective B cell maturation, was compared to phenotypically normal F1 female mice. B lymphocytes of F1 males exhibit an abnormal mitogenic response to LPS [lipopolysaccharide] in serum-free culture conditions, which is partially reversed in the presence of serum. Resident and thioglycollate-induced peritoneal macrophages of F1 male mice respond normally to LPS. In response to LPS in vitro, F1 male macrophages produce the monokine, lymphocyte-activating factor (LAF) and release prostaglandins. F1 male macrophages are sensitive to the lethal effects of LPS. Therefore, the defective CBA/N gene appears to be expressed only in B lymphocytes and not in macrophages. Since F1 male mice are normally sensitive to the lethal and adjuvant effects of LPS in vivo, a mature B lymphocyte population is probably not required for these effects. The role of the macrophage in the mediation of LPS-induced lethality and adjuvanticity is supported.

This publication has 12 references indexed in Scilit: