MUTATION-INDUCTION BY 5-FLUORODEOXYURIDINE IN SYNCHRONOUS CHINESE-HAMSTER CELLS
- 1 January 1979
- journal article
- research article
- Vol. 39 (3) , 808-810
Abstract
Chinese hamster ovary CHO cells synchronized by mitotic detachment were treated with 5-fluorodeoxyuridine [an antineoplastic drug] (FdUrd), 0.2 .mu.g/ml, at various times in the cell cycle. FdUrd treatment in the early S period induced resistance to 6-thioguanine. Treatment of asynchronous cells with FdUrd caused little increase in 6-thioguanine resistance over the spontaneous frequency. Mitotic selection thus enhances the probability of a cell being in the portion of S period receptive to mutation. FdUrd treatment may lead to mutagenesis at the growing points of DNA replication.This publication has 7 references indexed in Scilit:
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