An Improved Method for Routine Analysis of Polyamines in Biological Fluids with a Conventional Amino Acid Analyzer

Publisher Website
Google Scholar
Abstract
An accurate and precise mechanized ion-exchange chromatographic method for the estimation of polyamines in biological fluids [plasma or urine] is described. The method includes the recent improvements developed for ion-exchange analysis of polyamines: the use of sulfonated polystyrene cation-exchange resin with a bead diameter of < 10 .mu.m and a continuous fluorescent detection with o-phthalaldehyde. Four different procedures for the removal of protein before analysis of polyamines in plasma were compared. Linearity of the fluorescence detection, reproducibility (intra-, interassay) and precision of the retention times are reported. The method is characterized by the simplicity of the sample preparation, a clear and rapid elution program and especially by its routine applicability.

This publication has 16 references indexed in Scilit: