Effects of Feeding Autoclaved Pancreas to Depancreatized and Duct Ligated Dogs

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Abstract
The majority of insulin-treated depancreatized dogs develops a syndrome characterized by a progressive decrease in body wt. and disappearance of body fat, a progressive increase in alkaline phosphatase of the serum, a decrease in the amt. of insulin required to prevent glycosuria, a progressive decrease in concn. of the blood lipids, the development of fatty infiltration of the liver with impairment of liver function, and an increase in sensitivity to insulin so that hypoglycemia convulsions are difficult to prevent. These findings likewise develop in the majority of dogs whose pancreatic ducts were ligated and the parenchyma of the pancreas destroyed except that most of these animals do not require insulin. This deficiency syndrome may be completely prevented or relieved by oral admn. of fresh pancreas or of pancreas that has been heated in the autoclave at 120[degree]C and 15 lbs. pressure for from 30-60 min. until proteolytic enzymes present have been largely destroyed or inactivated. Liver and salivary glands similarly treated exhibit no beneficial effect. These findings support the conclusion that the active principle in pancreas, lipocaic, is a specific substance present in pancreas but not in other tissues.

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