The Precipitation of Purified Concentrated Influenza Virus and Vaccine on Calcium Phosphate

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Abstract
Formalinized influenza virus purified by a single cycle of differential centrifugation was obtained readily at a conc. of about 3 mg. per ml. and was thus useful in preparing virus-Ca phosphate ppts. in which the ratios of virus to Ca phosphate could range from about 1:2 to any larger value. More concentrated virus yielding ratio down to 1:1 were obtainable. The immunizing potency of different amts. of ppts. containing various virus-Ca phosphate ratios must be detd. to establish the optimum ratio and amt. of ppt. needed for human immunization. Since ratios less than about 1:25 were not obtained with allantoic fluid, whereas with conc. virus any ratio down to about 1:1 was obtainable, a much more complete study of the adjuvant effect of Ca phosphate on influenza virus vaccines is possible by the use of virus materials conc. and purified by differential centrifugation.