Primers with 5′ Flaps Improve Real-Time PCR
Open Access
- 1 December 2007
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 43 (6) , 770-774
- https://doi.org/10.2144/000112631
Abstract
Primers that contain portions noncomplementary to the target region are usually used to add to the PCR product a utility sequence such as a restriction site or a universal probe binding site. We have demonstrated that primers with...Keywords
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