m‐Iodobenzylguanidine increases the mitochondrial Ca2+ pool in isolated hepatocytes
- 16 November 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 313 (1) , 39-42
- https://doi.org/10.1016/0014-5793(92)81179-p
Abstract
The incubation of isolated hepatocytes with the inhibitor of protein mono ADP‐ribosylation, m‐iodobenzylguanidine (MIBG), resulted in an increase in the size of the mitochondrial Ca2+ pool, without alteration of the non‐mitochondrial Ca2+ store(s). This increase was abolished when the cytosolic free Ca2+ concentration ([Ca2+]i) was buffered by prior loading of the cells with fluo 3. Elevating [Ca2+]i by releasing the endoplasmic reticular Ca2+ store with 2,5‐di‐(tert‐butyl)‐1,4‐hydroquinone resulted in a synergistic increase in the magnitude of the mitochondrial Ca2+ pool. A role for protein ADP‐ribosylation in the intracellular regulation of mitochondrial Ca2+ homeostasis is suggested.Keywords
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