Association of eukaryotic DNA topoisomerase I with nucleosomes and chromosomal proteins
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (2) , 461-472
- https://doi.org/10.1093/nar/11.2.461
Abstract
A DNA topoisomerase activity is found to be associated with the nucleosomes released by the Staphylococcal nuclease digestion of HeLa nuclei. Such an association is found to be salt dependent. A number of criteria have established that this DNA topoisomerase activity is due to HeLa topo I (Liu, L. F. and Miller, K. G. (1980) Proc. Natl. Acad. Sci. USA 78, 3489-3491). A similar association has been demonstrated from the in vitro studies using purified mononucleosomes and eukaryotic DNA topoisomerase I. Nonhistone HMG proteins and histone H1 are found to stimulate topoisomerase activity in vitro and form tight complexes with eukaryotic DNA topoisomerase I. The intimate interactions of topoisomerase I with chromosomal proteins and nucleosomes may be an essential feature of the topoisomerase function in vivo.Keywords
This publication has 22 references indexed in Scilit:
- Escherichia coli DNA topoisomerase I catalyzed linking of single-stranded rings of complementary base sequencesNucleic Acids Research, 1978
- Proteins that Affect DNA ConformationAnnual Review of Biochemistry, 1978
- Physicochemical studies of non-histone protein HMG17 with DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977
- Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.Proceedings of the National Academy of Sciences, 1977
- The Primary Structure of a Non-histone Chromosomal ProteinEuropean Journal of Biochemistry, 1977
- Histones H2A and H2B are neighbors along DNA in chromatin: characterization of subnucleosomal particles containing H2A+H2BNucleic Acids Research, 1977
- [45] Reductive alkylation of amino groupsPublished by Elsevier ,1977
- An improved large scale fractionation of high mobility group non-histone chromatin proteinsBiochimica et Biophysica Acta (BBA) - Protein Structure, 1975
- Interaction of a Non‐Histone Chromatin Protein (High‐Mobility Group Protein 2) with DNAEuropean Journal of Biochemistry, 1975
- Interactions of a Purified Non‐Histone Chromosomal Protein with DNA and HistoneEuropean Journal of Biochemistry, 1974