Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation.
- 25 October 1994
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (22) , 10502-10506
- https://doi.org/10.1073/pnas.91.22.10502
Abstract
Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG.Keywords
This publication has 36 references indexed in Scilit:
- Coordinate suppression of mutations caused by Robertson's mutator transposons in maize.1994
- Differences in DNA‐methylation are associated with a paramutation phenomenon in transgenic petuniaThe Plant Journal, 1993
- Allelic interactions at the nivea locus of Antirrhinum.Plant Cell, 1991
- Differential inactivation and methylation of a transgene in plants by two suppressor loci containing homologous sequencesPlant Molecular Biology, 1991
- Transformation of a partial nopaline synthase gene into tobacco suppresses the expression of a resident wild-type gene.Proceedings of the National Academy of Sciences, 1991
- PREMEIOTIC INSTABILITY OF REPEATED SEQUENCES IN NEUROSPORA CRASSAAnnual Review of Genetics, 1990
- The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformantsPlant Molecular Biology, 1990
- Genomic footprinting reveals cell type-specific DNA binding of ubiquitous factorsCell, 1987
- Sequence of a genomic DNA clone for the small subunit of ribulose bis-phosphate carboxylase-oxygenase from tobaccoNucleic Acids Research, 1985
- Substrate and sequence specificity of a eukaryotic DNA methylaseNature, 1982