Stability Test of Six Enzymes for Internal Quality Control

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Abstract
The daily quality control for the determination of the catalytic activity concentrations of enzymes is an important aspect in clinical chemistry. Instead of the expensive, commercially available control sera, a simple, reliable and cheap method was sought for the quality control of enzyme determinations. Commercially available enzymes were suspended in an albumin solution and ampoules were filled with 1.0 ml of these various solutions. The ampoules were stored at 4.degree. C or -20.degree. C. Once a week, during 10 mo., catalytic activities of these enzyme-albumin solutions were determined together with the same activities in freshly reconstituted control sera. Aspartate aminotransferase [EC 2.6.1.1], alanine aminotransferase [EC 2.6.1.2], alkaline phosphatase [EC 3.1.3.1], creatine kinase [EC 2.7.3.2] and .gamma.-glutamyltransferase [EC 2.3.2.2] were determined at 30.degree. C according to well-described methods. .alpha.-Amylase [EC 3.2.1.1] was determined with the Phadebas method at 37.degree. C. Except for creatine kinase, the stability and reliability of these enzyme solutions are fully comparable with control sera during the experimental period. The catalytic activity concentration of creatine kinase decreased slowly during the 10 mo. The enzyme solutions react in the same manner as commercial test sera on changes in the reaction conditions for the enzyme determinations. The conclusion seems justified that these enzyme solutions can be used for the daily quality control of the enzyme determinations instead of control sera.

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