PLASMA CHOLECYSTOKININ RESPONSES AFTER INGESTION OF LIQUID MEAL AND INTRADUODENAL INFUSION OF FAT, AMINO-ACIDS, OR HYDROCHLORIC-ACID IN MAN - ANALYSIS WITH REGION SPECIFIC RADIOIMMUNOASSAY

  • 1 January 1983
    • journal article
    • research article
    • Vol. 78  (11) , 703-707
Abstract
A highly sensitive and precise radioimmunoassay system for plasma cholecystokinin (CCK) was developed with the anti-CCK-8 specific antiserum which raised against N-terminal amino acids residue of sulfated CCK-8 and reacted with CCK-8, CCK-33 and CCK-39 but not with gastrin and its related peptides. Mean concentration of the fasting plasma CCK determined with this method using CCK-8 as standard was 12.9 .+-. 5.9 pg/ml in normal subjects (n = 26), and in patients with hepatic cirrhosis it was significantly higher (36.7 .+-. 16.9 pg/ml, n = 9, P < 0.01) than in normal subjects. In 6 young healthy volunteers, intraduodenal infusion of fat caused a significant increase (P < 0.05) of plasma CCK from a basal level of 8.0 pg/ml to a peak of 43.0 .+-. 12.0 pg/ml at 20 min after starting of infusion. In the same subjects, a significant increase of plasma CCK was also observed by amino acids infusion, but no elevation of plasma CCK level was found during intraduodenal acidification.

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