HCS REGULATION IN CULTURED PLACENTA: ACTION OF GLUCOSE

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Abstract
The regulation of hCS [human chorionic somatomammotropin] secretion on placenta cultures in the presence of [3H]leucine was studied. When Eagle''s medium containing 0.0055 M glucose/l was used, the hCS increased regularly in the culture medium, whereas it diminished in the explants. [3H]hCS represented about 50% of the [3H]proteins, and among these proteins hCS were preferentially secreted in the culture medium. The absence of glucose in the medium provoked a decrease in all the cellular metabolism and a decrease in protein and hCS secretions, with an accumulation of preformed hCS in the tissues. An increase in the glucose level in the media, 0.011 and 0.027 M, provoked a selective decrease of hCS secretion with an accumulation of preformed hCS in the tissues, determining a long-term decrease in hCS synthesis. Insulin added in the media (60 .mu.U[unit]/ml) did not change the results significantly. The use of structural analogs of glucose showed that the point of impact of the hCS secretion regulation by the glucose is membranous. At a 2nd stage, 1 or more glucose metabolites may also intervene. There is no self-regulation of hCS secretion in vitro.

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