Detection of Human Papillomavirus DNA in Cervical Lesions by in Situ Hybridization Using Biotinylated DNA Probes
- 1 October 1990
- journal article
- research article
- Published by Wolters Kluwer Health in International Journal of Gynecological Pathology
- Vol. 9 (4) , 306-315
- https://doi.org/10.1097/00004347-199010000-00002
Abstract
Summary: In situ hybridization (ISH) for human papillomavirus (HPV)-6, -11, -16, -18, and -31 DNA was performed on 615 formalin-fixed paraffin-embedded cervical biopsies using biotinylated DNA probes. Results were obtained from 584 samples with 266 (45.5%) containing HPV-DNA sequences. Ninety percent of condyloma acuminatum specimens were positive for HPV-DNA with 18 of 19 positive cases containing HPV-6 or -11 DNA. The detection rate of HPV in cervical intraepithelial neoplasia (CIN) lesions was 50.6% (239 of 472), while only 8 of 91 (8.9%) cervical biopsies considered to be histologically normal or with minimal dysplasia contained HPV-DNA as demonstrated by ISH. The prevalence of HPV-16, -18, and/or -31 DNA increased with the severity of the lesions, with 20 of 20 (100%) positive CIN-III lesions containing these viral types compared with 102 of 157 (65.0%) positive CIN-I lesions. ISH with biotinylated DNA probes appears helpful in identifying lesions containing higher risk viral strains.Keywords
This publication has 26 references indexed in Scilit:
- DNA sequences of human papillomavirus types 11, 16 or 18 in invasive cervical carcinoma of Western Australian womenImmunology & Cell Biology, 1987
- DETECTION OF HUMAN PAPILLOMAVIRUS DNA IN INVASIVE CARCINOMAS OF THE CERVIX BY INSITU HYBRIDIZATION1987
- In situ hybridization detection of human papillomavirus DNAs and messenger RNAs in genital condylomas and a cervical carcinomaHuman Pathology, 1986
- Human Papillomavirus in Clinically and Histologically Normal Tissue of Patients with Genital CancerNew England Journal of Medicine, 1986
- DNA sequences of human papillomavirus types 11, 16, and 18 in lesions of the uterine cervix in the west of Scotland.BMJ, 1986
- Human papillomavirus (HPV) DNA sequences demonstrated by in situ DNA hybridization in serial paraffin-embedded cervical biopsiesArchiv für Gynäkologie, 1986
- INSITU HYBRIDIZATION ANALYSIS OF HPV 16 DNA-SEQUENCES IN EARLY CERVICAL NEOPLASIA1986
- Prevalence of human papillomavirus type 16 DNA sequences in cervical intraepithelial neoplasia and invasive carcinoma of the cervixBJOG: An International Journal of Obstetrics and Gynaecology, 1985
- Detection and localization of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probesJournal of Medical Virology, 1985
- OCCURRENCE OF HUMAN PAPILLOMAVIRUS TYPE-16 AND TYPES-18 DNA IN CERVICAL CARCINOMAS FROM JAPAN - AGE OF PATIENTS AND HISTOLOGICAL TYPE OF CARCINOMAS1985