Isolation of an intermediate of 2-deoxystreptamine biosynthesis from a mutant of Bacillus circulans.

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Abstract
Eight 2-deoxystreptamine-negative (DOS-) mutants were isolated from various strains of B. circulans, normally producing butirosin, xylostasin and ribostamycin. These mutants were classified into 2 groups (converter and secretor) by the simple method of cosynthesis using agar plate culture. One of the cosynthetic pairs, strain S-11 and strain 236, was selected for further study. From the fermentation broth of strain S-11, an intermediate of DOS biosynthesis, S-11-P, was isolated. This compound was converted to butirosin (BTN) effectively by strain 236. The structure of S-11-P was considered to be (1 L or 1 D)-1,3,5/2,4-5-aminocyclohexanetetrol and the pathway of DOS biosynthesis is discussed here.