Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P‐450 in rat adrenal cortex
Open Access
- 24 April 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 263 (2) , 299-302
- https://doi.org/10.1016/0014-5793(90)81398-8
Abstract
Using an oligonucleotide probe designed on the basis of the N‐terminal amino acid sequence of purified rat aldosterone synthase cytochrome P‐450 [(1989) J. Biol. Chem. 264 10935] we have isolated from rat adrenal cDNA library a 2687 base pair cDNA that encodes a protein of 500 amino acid residues. The deduced amino acid sequence contained the regions well conserved among all cytochrome P‐450s sequenced to date, and also a portion (residues 25–44) which was identical to the N‐terminal peptide sequence of rat aldosterone synthase cytochrome P‐450. These results indicate that the cDNA encodes a precursor form of rat aldosterone synthase cytochrome P‐450.Keywords
This publication has 11 references indexed in Scilit:
- Uncoupling of the cytochrome P-450cam monooxygenase reaction by a single mutation, threonine-252 to alanine or valine: possible role of the hydroxy amino acid in oxygen activation.Proceedings of the National Academy of Sciences, 1989
- Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P‐45011βFEBS Letters, 1989
- Isolation of Aldosterone Synthase Cytochrome P-450 from Zona Glomerulosa Mitochondria of Rat Adrenal CortexJournal of Biological Chemistry, 1989
- The 2.6-A crystal structure of Pseudomonas putida cytochrome P-450.Journal of Biological Chemistry, 1985
- Molecular cloning and nucleotide sequence of cDNA for mRNA of mitochondrial cytochrome P-450(SCC) of bovine adrenal cortex.Proceedings of the National Academy of Sciences, 1984
- Coding nucleotide sequence of 3-methylcholanthrene-inducible cytochrome P-450d cDNA from rat liver.Proceedings of the National Academy of Sciences, 1984
- A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.Molecular and Cellular Biology, 1983
- Amino acid sequence of an analogous peptide from two forms of cytochrome P-450.Journal of Biological Chemistry, 1981
- Plasmid screening at high colony densityGene, 1980
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977