High Levels of Anti‐Mitochondrial Antibodies in MRL Mice Influence of Anti‐DNA Antibodies on Anti‐Mitochondrial Antibodies Measured by an Enzyme‐Linked Immunosorbent Assay

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Abstract
We developed an easy enzyme‐linked immunosorbent assay (ELISA) for anti‐mitochondrial antibodies, and detected high levels of anti‐mitochondrial antibodies in MRL/Mp‐lpr/lpr (MRL 1/1) mice. The influence of the presence of anti‐DNA antibodies in the tested sera on this assay was also evaluated. The monoclonal anti‐DNA antibodies, which were made from non‐immunized MRL 1/1 mice, did not react with the mitochondrial antigens adhering to polystyrene plates. Absorption with DNA had little effect on the levels of mitochondrial antibodies in MRL 1/1 sera. Our assay for mitochondria‐binding antibody was very easy and sensitive, although it could not differentiate among heterogeneous anti‐mitochondrial antibodies.