Solubilization of four Porcine Zona pellucida Antigens by Lithium 3,5-Diiodosalicylate

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Abstract
The lack of detailed studies of zone pellucida antigens up to the present time is partly due to the difficulty of the purification procedures involved. Four porcine zona pellucida antigens were isolate using lithium 3,5-diiodosalicylate as the solubilizing agent and immuno-affinity chromatography, with immobilized IgG, for purification. This method is believed to be more specific than the previous techniques used. The 4 zona antigens, ZP I/1 (MW = 42,000), ZP II/1 (MW = 67,000), ZP II/2 (MW = 32,000) and ZP III/1 (MW = 17,000) were identified. Based on the reaction of the antigens with antibodies induced by intact and heat-solubilized zonae, it is postulated that only ZP I/1 and ZP II/1 are expressed on the surfaces of intact zonae. The cross reaction between human and porcine zona pellucida antigens is attributed to ZP I/1.