EXPERIMENTAL MANIPULATION OF ALTERNATIVE PATHWAYS OF DIFFERENTIATION IN CULTURES OF EMBRYONIC CHICK NEURAL RETINA

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Abstract
Embryonic chick neural retina cells can transdifferentiate during long-term cell culture into pigmented epithelium or lens fibers. Some culture conditions influence the choice between these pathways. Pigment cell development was promoted by low initial cell densities and by the use of a medium based on Earle''s salt formulation rather than Hank''s, while lens fiber development was encouraged by high initial cell densities and by folding the cell sheet into multilayered regions. Some differences in in vitro cell properties of neural retina were reported for 2 genotypes previously found to exhibit differences in in vitro cell properties of lens epithelial cells.