A DEMONSTRATION OF LENS FORMING-CELLS IN NEURAL RETINA IN CLONAL CELL CULTURE

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Abstract
Clonal cultures with 1000-3000 cells were prepared from cells harvested from high density cultures of neural retina of 8 day old chick embryos. About 1.14% and 0.31% of inoculated cells developed into recognizable colonies in Eagle''s MEM [minimal essential medium] and in Ham''s F-12 supplemented with fetal calf serum, respectively. Lentoid bodies of authentic lens nature were differentiated in 10% and 33.52% in MEM and F-12, respectively. Cells harvested from high density cultures of the anterior and posterior portions of the neural retina were clonally cultured. Plating efficiency was much higher in the anterior cells than in the posterior ones and clones with lentoid differentiation were developed only in clonal cultures of the anterior cells.