Abstract
The starting material was lipoid free beef erythrocyte stroma. This was treated with boiling 2% methanolic HC1 which brought the neuraminic acid into solution. This was then converted to the methoxy-derivative. Purification was accomplished first on a cellulose column, then by paper chromatography, and the pure methoxy neuraminic acid was finally crystallized from alcohol and recrystallized from water. Elementary analyses confirmed the identity of the isolated substance with methoxy-neuraminic acid.