ACAPELLA-1K, a capillary-based submicroliter automated fluid handling system for genome analysis.
- 1 January 2000
- journal article
- Vol. 10 (1) , 95-104
Abstract
The Genomation Laboratory in the Electrical Engineering Department at the University of Washington has been developing an automated, high-throughput, submicroliter-scale fluid-handling system for use in molecular biology, especially as part of the Human Genome Project and other high-throughput DNA sequencing endeavors. Small glass capillaries enable the preparation, handling, and monitoring of 1-microliter reaction volumes. The Genomation Laboratory, with corporate partners Orca Photonic Systems, Inc. and Engineering Arts, has developed modules for aspiration, dispensing, mixing, transport, and rapid thermal processing of biological samples contained in glass capillaries. The ACAPELLA-1K is the first integration of these modules, designed to process 1000 samples in an eight-hour day. It has served as a test bed for the technologies as well as for performing biological experiments in conjunction with the University of Washington Genome Center. This system and related results are presented in this paper. A video of the system in operation is provided at. The Genomation Laboratory is presently developing the next-stage ACAPELLA-5K system based on the results of the ACAPELLA-1K system.This publication has 17 references indexed in Scilit:
- High-Density Small-Volume Gel Loading Directly from Capillary TubesBioTechniques, 1999
- An Automated Sample Preparation System for Large-Scale DNA SequencingGenome Research, 1999
- Nanoliter scale PCR with TaqMan detectionNucleic Acids Research, 1997
- Chip PCR. II. Investigation of different PCR amplification systems in microbabricated silicon-glass chipsNucleic Acids Research, 1996
- Large-Scale and Automated DNA Sequence DeterminationScience, 1991
- Minimizing the time required for DNA amplification by efficient heat transfer to small samplesAnalytical Biochemistry, 1990
- Automated polymerase chain reaction in capillary tubes with hot airNucleic Acids Research, 1989
- Automated DNA sequencing and analysis of the human genomeGenomics, 1987
- The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysisNucleic Acids Research, 1985
- Characterization of an unusual DNA length polymorphism 5' to the human antithrombin III geneNucleic Acids Research, 1983