An Automated Sample Preparation System for Large-Scale DNA Sequencing
- 1 May 1999
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 9 (5) , 457-462
- https://doi.org/10.1101/gr.9.5.457
Abstract
Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day from libraries of M13 plaques at a cost of $0.29 per sample. This Front End has been in high throughput operation since June, 1997 and has produced > 400,000 high-quality DNA templates.Keywords
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