Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells
- 1 April 1982
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 296 (5856) , 404-409
- https://doi.org/10.1038/296404a0
Abstract
DNA from T24, a cell line derived from a human bladder carcinoma, can induce the morphological transformation of NIH 3T3 cells. Using techniques of gene rescue to clone the gene responsible for this transformation, it was found to be human in origin, < 5 kilobase pairs in size and homologous to a 1100-base polyadenylated RNA species found in T24 and HeLa [cervical carcinoma] cells. Blot analysis indicates extensive restriction endonuclease polymorphism near this gene in human DNAs.Keywords
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