A Comparison Between a Micro and an Ultramicro Circle®Cell for on-Line Ft-Ir Detection in a Reverse Phase HPLC System
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 14 (2) , 377-393
- https://doi.org/10.1080/01483919108049622
Abstract
Caffeine and theophylline peaks separated on a 300 × 7.8 mm C18 column could be distinguished qualitatively by FT-IR detection using two types of cylindrical internal reflectance (CIRCLE®) cells. Detection limits and limits of identification, on the order of 0.2%, are lower for the micro CIRCLE® cell as compared to the ultramicro CIRCLE® cell. Maximum sample capacity of the column used was 50uL of a 0.4% solution of caffeine (1.0 micromoles).Keywords
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