The Bacteriophages of Pseudomonas pyocyanea: 2. Bacteriophage Reproduction in an Iridescent Strain

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Abstract
Two new compounds inhibited the increase of phage when 2 phages of P. pvocvanea were grown on an indicator strain. These compounds also inhibited the appearance of iridescence in a lysogenic strain of this organism. One iridescent lysogenic strain showed a rise in phage titer at 1-2 hrs., which corresponded to the latent period of the phage when it acted on an indicator strain. Both these increases were inhibited by the compounds. This strain also showed a rise in titer at 4 hrs. but this was unaffected by the compounds. The final phage titer of the lysogenic culture was not affected significantly, unlike the complete and permanent suppression of phage increase when free phage acted on an indicator strain. Cultures and filtrates of three lysogenic strains of P. pvocvanea showed a great increase in phage titer when assay plates were incubated for a further period after the initial reading at 18 hrs.

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