LYMPHOKINE (INTERLEUKIN 2) SECRETION AS A MEASURE OF T CELL RECOGNITION OF PARASITE ANTIGENS

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Abstract
The secretion of the lymphokine. Interleukin 2 (IL2), from cells obtained from the lymph nodes of mice infected by the murine nematode. Nematospiroides dubius, is used an indication of T cell recognition of parasite antigens. It is demonstrated that IL2 secretion is T cell-dependent and occurs only when the lymph node cells are cultivated in the presence of at least 5 g/ml of a parasite antigen preparation. The presence of adherent peritoneal exudate cells from uninfected mice in the cultures is an essential requirement for lyraphokine release. This system can be used to quantitate T cell recognition of parasite antigens and to study T cell/antigen-presenting-cell interactions involved in the immune response to parasite infection.