Isolation of human lactate dehydrogenase isoenzyme X by affinity chromatography
- 1 September 1978
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 173 (3) , 767-771
- https://doi.org/10.1042/bj1730767
Abstract
Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. In the presence of 1.6 mM-NAD+, isoenzyme LDH-X does not bind to AMP-Sepharose, whereas the other lactate dehydrogenase isoenzymes do. This is the crucial point in the isolation of isoenzyme LDH-X from the other isoenzymes. The purified human isoenzyme LDH-X had a specific activity of 146 units/mg of protein.Keywords
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