Studies on vitamin E. 5. Lipid peroxidation in dialuric acid-induced haemolysis of vitamin E-deficient erythrocytes

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Abstract
Dialuric acid-induced haemolysis of vitamin E-deficient erythrocytes has been shown to be accompanied by lipid peroxidation. Formation of peroxides is prevented by agents protecting erythrocytes against haemolysis (a-tocopheroL 2:6-ditert.-butyl-4-methylphenol and the ions Co2+, Mn2+, Cr2O72- and CrO42-). The protection of a-tocopherol is completely opposed by SeO32-ions. Studies with L-thyroxine have shown it to be fully protective in vitro against both haemolysis and lipid peroxidation. Haemoglobin catalysis of lipid peroxidation in the erythrocyte stroma has been demonstrated by cell-fractionation studies and by the protection of intact cells resulting from the conversion of oxyhaemoglobin into carboxyhaemoglobin. From the observed effects of excess of dialuric acid, it seems that oxyhaemoglobin may dissociate during haemolysis, but only to a small extent.