Electron Microscope Autoradiography Of A Diffusible Intracellular Constituent, Using Freeze‐Dried Frozen Sections Of Mammalian Intestinal Epithelium

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Abstract
Autoradiograms were prepared from freeze‐dried frozen sections of rat intestinal epithelium, previously incubated in vitro with 3H‐galactose. Although the sections snowed evidence of disruption by ice‐crystal growth during freezing, they retained sufficient recognizable ultrastructure to facilitate the identification of several subcellular compartments. Statistical analysis of the grain distribution indicated that a significant non‐uniform distribution of the labelled galactose occurs in the absorptive cells during transport, and is maintained in the sections throughout the various stages of autoradiography.