Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method
Top Cited Papers
- 31 January 2007
- journal article
- Published by Springer Nature in Nature Protocols
- Vol. 2 (1) , 35-37
- https://doi.org/10.1038/nprot.2007.14
Abstract
Here, we describe a quick and easy version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method can be performed when only a few transformants are needed. The procedure can take less than an hour, depending on the duration of the heat shock. It can be used to transform yeast cells from various stages of growth and storage. Cells can be transformed from freshly grown cells as well as cells stored on a plate at room temperature or in a refrigerator.Keywords
This publication has 7 references indexed in Scilit:
- High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG methodNature Protocols, 2007
- 4 Transformation of Yeast by the Lithium Acetate/Single-Stranded Carrier DNA/PEG MethodPublished by Elsevier ,1998
- Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedureYeast, 1995
- High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrierCurrent Genetics, 1989
- [53] Isolation of genes by complementation in yeastPublished by Elsevier ,1987
- Transformation of intact yeast cells treated with alkali cationsJournal of Bacteriology, 1983