High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

Top Cited Papers

31 January 2007

journal article
research article
Published by Springer Nature in Nature Protocols

Vol. 2 (1) , 31-34
https://doi.org/10.1038/nprot.2007.13

Abstract

Here we describe a high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae. This method currently gives the highest efficiency and yield of transformants, although a faster protocol is available for small number of transformations. The procedure takes up to 1.5 h, depending on the length of heat shock, once the yeast culture has been grown. This method is useful for most transformation requirements.

Keywords

This publication has 11 references indexed in Scilit:

Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method
Nature Protocols, 2007
Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method
Nature Protocols, 2007
Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method
Nature Protocols, 2007
Genetic Transformation of Yeast
BioTechniques, 2001
Stabilizing Amino Acid Replacements at Position 52 in Yeast Iso-1-cytochrome c: In Vivo and in Vitro Effects
Biochemistry, 1995
Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
Yeast, 1995
Parameters affecting the frequencies of transformation and co‐transfromation with synthetic oligonucleotides in yeast
Yeast, 1992
High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier
Current Genetics, 1989
[53] Isolation of genes by complementation in yeast
Published by Elsevier ,1987
Transformation of intact yeast cells treated with alkali cations
Journal of Bacteriology, 1983