Measurement of Hydrolytic Activity of Lipase by Isooctane Addition System

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Abstract
An isooctane-addition agitated method is newly proposed for the assay of the hydrolytic activity of lipase. The hydrolytic activities of six kinds of microbial lipase were measured with this method at a high lipase concentration range and using olive oil, palm oil, and beef tallow as substrates. The newly proposed method gave equivalent or higher values than the previously proposed methods. In addition, the activity values calculated from the initial slopes of the progress curves gave a good proportional relation between enzyme concentration and activity in a wide range, although those calculated from the data obtained only at a fixed time (20 min) were out of proportion to the enzyme concentration by all methods tested.

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