Untersuchungen am Prothrombin-Komplex, I. Das Verhalten von Prothrombin, Faktor VII, IX und X an Bariumsulfat

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Abstract
Factors of the ''prothrombin complex'' may be adsorbed onto barium sulphate. The conditions that stimulate or inhibit this and the reverse process of desorption were investigated. Ca inhibits the adsorption. In the presence of Ca binding anticoagulants, there axe 2 competing reactions: removal of the Ca, which inhibits adsorption; affinity for the barium sulphate. Competition occurs at the binding sites on the barium sulphate which adsorb coagulation factors. Neutral salts, even at high concentration, have no influence on adsorption. Although all parameters were varied to give optimal conditions, it was not possible to separate the plasma factors prothrombin, factor VIL IX and X, and the serum factors VII, IX and X from one another.