Production of Human IgM Anti‐D in Tissue Culture by EB‐Virus‐transformed Lymphocytes
- 1 October 1980
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 12 (4) , 355-358
- https://doi.org/10.1111/j.1365-3083.1980.tb00077.x
Abstract
Lymphocytes obtained from two donors who were producing anti‐D were transformed with Epstein‐Barr virus and grown in tissue culture. The lymphoblasts from both donors produced anti‐D antibody, which agglutinated Rh‐positive cells in saline. Both antibodies belonged to the IgM class; the concentration of anti‐D in the culture fluid from one of the donors was approximately 250–500 ng/ml and bound firmly to Rh‐positive cells with a functional affinity constant of approximately 1 × 109 M−1.This publication has 7 references indexed in Scilit:
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