Isolation and Characterization of Temperature-sensitive Mutants of Abelson Murine Leukaemia Virus that Exhibit Dissociation among Morphological Transformation, Soft Agar Colony-forming Ability and Tyrosine Kinase Activity
- 1 September 1989
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 70 (9) , 2527-2532
- https://doi.org/10.1099/0022-1317-70-9-2527
Abstract
Summary Seven temperature-sensitive (ts) mutants of Abelson murine leukaemia virus (A-MuLV) were isolated on the basis of the temperature dependence of their soft agar colony-forming ability. These seven ts mutants exhibited similar characteristics and were not ts for morphological transformation and autophosphorylation of P120gag-abl protein. The dissociation of the properties of morphology, soft agar colony formation and tyrosine kinase activity might suggest that the v-abl product has more than one primary intracellular target.Keywords
This publication has 10 references indexed in Scilit:
- Isolation of temperature-sensitive Abelson virus mutants by site-directed mutagenesis.Proceedings of the National Academy of Sciences, 1987
- A temperature-sensitive mutant of Abelson murine leukemia virus confers inducibility of IgM expression to transformed lymphoid cells.The EMBO Journal, 1987
- Differentiation of an Abelson virus-transformed immature B precursor cell line under the expression of tyrosine kinase activity of v-abl oncogene productCell Differentiation, 1987
- Isolation of temperature-sensitive tyrosine kinase mutants of v-abl oncogene by screening with antibodies for phosphotyrosine.Proceedings of the National Academy of Sciences, 1987
- Temperature-sensitive membrane association of pp60src in tsNY68-infected cells correlates with increased tyrosine phosphorylation of membrane-associated proteinsVirology, 1983
- Relationship between the rearrangement of immunoglobulin genes, the appearance of a B lymphocyte antigen, and immunoglobulin synthesis in murine pre-B cell lines.The Journal of Immunology, 1982
- Biological properties of "partial" transformation mutants of Rous sarcoma virus and characterization of their pp60src kinaseJournal of Virology, 1981
- Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosineNature, 1980
- Dissociation of transformation parameters using temperature-conditional mutants of Rous sarcoma virusCell, 1979
- Distinguishable transformation-defective phenotypes among temperature-sensitive mutants of Rous sarcoma virusJournal of Virology, 1977