Herpes simplex virus replication in pheochromocytoma cell line that responds to nerve growth factor

Abstract
Cultured cells of neural origin (PC-12, pheochromocytoma cell line) respond to nerve growth factor (NGF) by extending neurites. These cells whether treated with NGF or not can be infected with herpes simplex virus and produce progeny virus. Viral antigens are detected on the cell surface and fusion of cells to form polykaryocytes takes place. Nucleocapsids are found within the cell nucleus and enveloped virus in present both in the cytoplasm and extracellular space. Virus was not observed within the neurites but alterations in the neurite microtubular structure occurred after infection.