Release of neuropeptides during intracellular stimulation of single identified Aplysia neurons in culture.
Open Access
- 1 December 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (24) , 9794-9798
- https://doi.org/10.1073/pnas.83.24.9794
Abstract
An important criterion for classifying a substance as a neurotransmitter is that it is released in an activity-dependent fashion. We have utilized cell culture of individual neurons of Aplysia to demonstrate the release of the neuropeptides SCPA and SCPB (small cardioactive peptides A and B). Neurons B1 and B2 were isolated from the buccal ganglion of Aplysia and maintained in cell culture. The cells grew new processes, which were immunoreactive to antibodies for the neuropeptide SCPB. These processes contained SCPA and SCPB that were detectable by bioassay on snail heart. The cells synthesized the SCPs from radiolabeled precursors and transported the peptides to their neurites. Single cells released SCPs in a calcium-dependent fashion upon intracellular electrical stimulation. Taken together, these results provide critical evidence that SCPs are neurotransmitters. The results also indicate that the cell culture of individual identified neurons can be used to investigate the release of peptides.This publication has 16 references indexed in Scilit:
- The effects of small cardioactive peptide B on the isolated heart and gill of Aplysia californicaCanadian Journal of Physiology and Pharmacology, 1985
- The small cardioactive peptides A and B of Aplysia are derived from a common precursor molecule.Proceedings of the National Academy of Sciences, 1985
- Intracellular Stimulation of an Identified Neuron Evokes Cardioacceleratory Peptide ReleaseScience, 1985
- Two endogenous neuropeptides modulate the gill and siphon withdrawal reflex in Aplysia by presynaptic facilitation involving cAMP-dependent closure of a serotonin-sensitive potassium channel.Proceedings of the National Academy of Sciences, 1984
- Neuropeptides: Mediators of Behavior in AplysiaScience, 1984
- Evidence for parallel actions of a molluscan neuropeptide and serotonin in mediating arousal in Aplysia.Proceedings of the National Academy of Sciences, 1984
- Peptide Cotransmitter at a Neuromuscular JunctionScience, 1983
- Elucidation by FAB–MS of the structure of a new cardioactive peptide from AplysiaNature, 1982
- Enkephalin inhibits release of substance P from sensory neurons in culture and decreases action potential duration.Proceedings of the National Academy of Sciences, 1979
- Intrasomatic Injection of Radioactive Precursors for Studying Transmitter Synthesis in Identified Neurons of Aplysia californicaProceedings of the National Academy of Sciences, 1973